Economic viability of the shale gas boom in the US Literature review

Economic viability of the shale gas boom in the US - Literature review Example economy, environment as well as political status in the future. Natural gas is known of reducing air pollution as a result of using coal by 30 percent (Energy Information Administration 2009 and Energy Information Administration (US) 2009). As a result of this, majority of the people believe that the use of natural gas will aid reduce pollution of air by fossil fuels such as coal and oil. The production and utilization of domestic fuels will have a significant impact on the economy of any state by creating job opportunities as well as increasing income through royalty payments (Energy Information Administration (US) 2011 and Schlumberger 2011). Additionally, the use of natural gas will enable the U.S. utilize the money used to be spent on fuel imports on other valuable goods (Energy Information Administration (US) 2010 and Energy Information Administration (US) 2011b). Although there are many shale gas reserves in the U.S., the most important is Marcellus Shale (Bloomberg 2011). Marc ellus Shale is located in Pennsylvania as well as Upstate New York. It has gained significant attention because of its closeness to most populated areas, its huge deposit size, and the amount of recoverable gas it has. Some of the factors that affect the productivity of the Marcellus Shale include â€Å"the drop in natural gas prices, overproduction claims, a decade of rising costs and apparent production declines in the shale gas wells† (Energy Information Administration 2012b). Economic analysis of this circumstance will enable the examination of the profitability of the shale gas well in the Marcellus Shale through looking at production as well as variables (Schoen 2011). The results gotten will then aid in evaluating whether producers are in a position to profitably extract natural gas despite the current market conditions. Profitability will then be measured by calculating crucial metrics such as internal rate of return of the chosen

Marketing Audit Essay Example | Topics and Well Written Essays - 750 words

Marketing Audit - Essay Example This paper will look at three components of the marketing audit namely marketing environment, marketing strategy and SWOT analysis. The marketing environment of a business organization can be classified into the macroenvironment and task environment. While the macroenvironment deals with the larger external environment of the company, the task environment looks at the direct stakeholders. The company's macroenvironment is comprised of the demographic, economic, environmental, technological, political, and cultural factors which directly affects its operation. Business organizations need to know the major demographic trends and developments which pose threats and opportunities to them as well as their specific response to these factors. The effects of economic variables such as price, income, savings and credit in the operation of business organizations should also be ascertained. ... Lastly, cultural factors like lifestyle and values must be evaluated to create products and services which are more preferred by customers (Kotler 2003). The task environment is comprised of the companies' stakeholders like suppliers, distribution channels, customers, competitors, customers, facilitators and marketing firms, markets, and publics. An evaluation of the task environment gives the business organization an idea of its position in the market (Kotler 2003). Marketing Strategy The evaluation of the company's marketing strategy start with the identification of the broader business mission and marketing objectives and goals. The main focus of this analysis is to determine whether the strategy is in line with where the company wants to be and what it wants to achieve. In evaluating the company's business mission, managers ascertain whether their stated mission is market-oriented. Market orientation is now imperative due to the evolving hypercompetitive marketing environment which gives higher buying leverage to customers. Next, the company also needs to verify that the company's stated business mission is feasible, that is, the company has the adequate and appropriate resources to achieve this position (Kotler 2003). Next, the company reviews its specific marketing objectives and goals making sure that they are smart-specific, measurable, attainable, realistic, and timely. Managers also ensure that the marketing objectives are appropriate given the company's position, resources, and opportunities. Lastly, the company turns to its marketing strategy taking note if it is really effective in achieving the stated goals and objectives. The company's marketing strategy is often relayed through its marketing

Adelphia Scandal and Worldcom Scandal Essay Example for Free

Adelphia Scandal and Worldcom Scandal Essay Basic Questions 1. Rigas Entities were entities that shared a common cash management system with Adelphia and Adelphia subsidiaries, which Adelphia controlled and operated. Since the scandal broke, it is commonly referred as off-the-book entities. 2. EBITDA (Earnings Before Interest, Taxes, Depreciation and Amortization) is essentially net income with interest, taxes, depreciation, and amortization added back to it, and can be used to analyze and compare profitability between companies and industries because it eliminates the effects of financing and accounting decisions.-from Investopedia 3. Self-dealing basically refers to when directors of a company improperly uses company finances or resource for personal gain. This can include directors taking company loans that the directors do not intend to repay, using company money for extraordinary personal use, or using company property for personal gain. See more: Is the Importance of being earnest a satirical play essay Advanced Questions 1. Both Adelphia scandal and WorldCom scandal were not prevented by company’s external auditor, though Deloitte and Touche and Arthur Andersen both rated their client as high risk. As for the differences, Adelphia did not have an independent internal auditor. However, WorldCom had an independent internal auditor and blows the whistle. 2. I will say Deloitte and Touche is most responsible for not detecting and stopping. As an external auditor, they should pay attention to organizations financial records and examine on any mistakes or fraud. At least, Deloitte and Touche should have stopped Timothy Rigas from serving as CFO and Director of Adelphia’s Accounting Committee. After all, it was obviously against the rules. 3. Timothy Rigas received a reasonable prison sentence as we can see from the WorldCom case; Bernard Ebbers was sentenced to 25 years. As for John Rigas, a former CEO who was guilty of more than 15 counts of fraud. Rationally speaking, it seems to be a fair judgment. However, it sounds too rough to keep an old man who has been suffered from cancer in jail.

Chromium Induced Toxicity Research

Chromium Induced Toxicity Research Abstract In the present study, we hypothesize that cytotoxicity, genotoxicity and oxidative stress play a key role in chromium induced toxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines when exposed for 24 h. Acute toxicity tests were conducted on three fish species namely L. calcarifer, E. suratensis and C. catla by exposing them to different concentration (0, 10, 20, 30, 40 and 50 mg/L) of chromium for 96 h under static conditions and the LC50 was calculated. The percentage cell survival was assessed by multiple endpoints such as MTT, NR, AB and CB assays were performed in seven fish cell lines exposed to different concentrations of chromium and EC50 values of all the four endpoints was calculated. Linear correlations between each in vitro cytotoxicity assay and the in vivo mortality data were highly significant. Microscopic examination of cell morphology indicated cell shrinkage, cell detachment, vacuolations and cell swelling at highest concentration of chromium (50mg/L). The DNA damage and nuclear fragmentation were assessed by comet assay and Hoechst staining, in seven fish lines exposed to different concentrations of chromium. The result of antioxidant parameter obtained show significantly decreased catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GSH) and Glutathione peroxidase (GPx), and increased level of lipid peroxidation (LPO) in all the cell lines after exposure to increasing chromium in a concentration-dependent manner. This results proves that fish cell lines could be used as an alternative to whole fish using cytotoxicity, genotoxicity and oxidative stress assessment after exposure to chromium. Keywords: Fish cell lines, Chromium, Cytotoxicity, Genotoxicity, Oxidative stress 1. Introduction Heavy metal pollution of water is a serious environmental problem facing the modern world. At global level heavy metals pollution is increasing in the environment due to increase in number of industries (Chidambaram et al. 2009). Industrial effluents are discharged into the sewage canals, rivers and irrigation water, causing major pollution and health hazards (Baddesha and Rao 1986). Many industrial wastewaters contain heavy metals like cadmium, lead, zinc, cobalt and chromium. The toxic heavy metals are mostly absorbed and get accumulated in various plant parts as free metals which may adversely affect the plant growth and metabolism (Barman and Lal 1994). Human beings and cattle are badly affected when these metals are incorporated into food chain as it causes bronchitis and cancer (Khasim et al. 1989; McGrath and Smith 1990; Nath et al. 2005). Among heavy metals, chromium plays a major role in polluting our aquatic environment system. In nature chromium occurs predominately in two valances Cr (III) and Cr (VI). Hexavalent chromium [Cr (VI)] predominates over the Cr (III) form in natural waters. Hexavalent chromium [Cr (VI)] particulates enter the aquatic medium through effluents discharged from leather tanning, textiles, chrome electroplating, metal finishing, dyeing and printing industries and several other industries. The Cr (VI) penetrates biological membranes easily and causes cellular damage by oxidative stress (Irwin et al. 1997; Begum et al. 2006), its unselective exposure may pose serious effect on aquatic communities including fish. Toxic effects of Cr(VI) on enzymological/biochemical (Al-Akel and Shamsi 1996; Vutukuru et al. 2007; Oner et al. 2008), hematological (Gautam and Gupta 1989; Al-Akel and Shamsi 1996), immunological (Prabakaran et al. 2007) parameters, endocrine toxicity (Mishra and Mohanty 2009) and genotoxicity (Chen et al. 2011) have been reported in many teleosts fishes. In environmental risk assessment, much of the toxicity test on fish has involved the use of lethality as the endpoint. On the other hand, in vivo bioassay is expensive and requires huge quantity of toxicant. The exposure time is only 24 h as opposed 96 h in bioassay, which could reduce the cost of labor, lab facilities and test time but more importantly allow decisions to be made more rapidly. Nevertheless, toxicity testing with fish is an essential part of environmental risk assessment procedures (Castano et al. 2003). For all these considerations, the development and use of in vitro assays that could measure early stages of toxicity in vertebrates represent an approach that could be very useful to monitoring environmental risk assessment (Walker 1999). Over the last four decades, cell and tissue culture methods have been refined and have now become an essential tool in environmental research. There are a lot of ethical, scientific and economical reasons that support the development of in vitro methods for use in ecotoxicology (Castano and Gomez-Lechon 2005; Bols et al. 2005; Schirmer, 2006; Fent 2007; Taju et al. 2012, 2013, 2014). The use of fish cell lines in environmental toxicology has been reviewed and positively assessed mainly with regards to cytotoxicity (Babich and Borenfreund 1991; Castano et al. 2003; Fent 2001). Cytotoxicity assessments can be readily employed to examine multiple endpoints, including measurements of cell death (apoptosis), cell viability, cellular morphology, cell metabolism, cell attachment/detachment, cell membrane permeability, proliferation, growth kinetics, genotoxicity and oxidative stress (Maracine and Segner 1998; Li and Zhang 2002; Shuilleabhain et al. 2004; Taju et al. 2014). In the present study, three fish species from three different aquatic environments, Lates calcarifer (Marine), Etroplus suratensis (Brackishwater) and Catla catla (freshwater) were selected as representatives of their respective environments to study their suitability for acute toxicity test to evaluate the potential risk of chromium (Cr). They are excellent food fishes with a good market demand in India, Malaysia, Bangladesh and Pakistan. Some attempts were made to study in vivo acute toxicity in Sea bass, Etroplus and Catla using various toxicants (Chezhian et al. 2010; Azmat and Javed 2011, 2012; Bhat et al. 2012; Taju et al. 2012, 2013). The seven fish cell lines namely SISK and SISS cell lines derived from L. calcarifer (Sahul Hameed et al. 2006; Parameshwaran et al. 2006b), SICH and ICG cell lines derived from C. catla (Ishaq Ahmed et al. 2009b; Taju et al. 2014), and IEE, IEK and IEG cell lines derived from E. suratensis (Sarath Babu et al. 2012) were used as in vitro assays t o evaluate the cytotoxicity, genotoxicity and oxidative stress exposed to chromium. The results of in vitro cytotoxicity were compared with the results of in vivo acute toxicity test using fish. The use of these cell lines for toxicity assessment of chromium instead of living fish is recommended. 2. Material and methods 2.1. Chemicals and reagents Tissue culture media and chemicals were obtained from GIBCO (Invitrogen Corporation, USA). Potassium dichromate (K2Cr2O7), EDTA, Trichloroacetic acid, DTNB [5,5-dithio-bis-(2-nitrobenzoic acid)], Thiobarbituric acid, Hydrogen peroxide, Nitro blue tetrazolium (NBT), Riboflavin, Hydroxylamine-HCl, Triton X-100, Ethidium bromide, Methanol, Acetic acid, Sodium chloride, Sodium hydroxide and Coomassie Blue was purchased from SRL chemicals, India. 2.2. Collection of experimental animals Lates calcarifer and Etroplus suratensis were collected from Central Institute of Brackishwater Aquaculture (CIBA), Chennai. Catla catla was collected from a local pond in Walajapet, Vellore District, Tamil Nadu, India. The experimental fishes were 2 3 g in body weight. Specimens were transported live in oxygen bags or buckets to the laboratory, acclimatized and maintained for 20-30 days in a salinity range of 5-10 ppt for E. suratensis, 20-25 ppt for L. calcarifer and in freshwater in the case of C. catla (23-28oC) under an ambient photoperiod in the laboratory for 10 days prior to experiments. The fish were fed with commercial pellet feed twice a day and starved for 24 h before and during the experiments. 2.3. In vivo fish acute toxicity test Fish acute toxicity tests were conducted by exposing E. suratensis, L. calcarifer and C. catla (N = 10 per aquarium) for 96 h to chromium under static conditions (OECD 203, 1992). Five different concentrations chromium i.e., 0, 10, 20, 30, 40 and 50 mg/L diluted with seawater (5 ppt) and freshwater while control with sea water and freshwater alone were tested to determine the LC50 (concentration at which 50% of the fish population dies). The aquaria had a working volume of 30 lit based on the body weight of fishes (1 g fish/L). Dead fishes were counted and removed immediately every day. All the experiments were conducted in triplicates. Mortalities were recorded following the guideline for fish acute toxicity OECD 203 (1992). 2.4. Fish Cell lines A total of seven cell lines established from different organs of L. calcarifer (SISS-seabass spleen, SISK-kidney), E. suratensis (IEE Etroplus eye, IEG gill, IEK kidney) and C. catla (SICH Catla heart, ICG gill) were tested for their sensitivities to chromium. These fish cell lines were propagated at 28oC in Leibovitzs L-15 medium (pH 7.0 -7.4) with 2mM L-glutamine, 10% foetal bovine serum (FBS), penicillin 100 IU/ml and streptomycin 100 ÂÂ µg/ml. The osmolarity ranged from 300 to 360 mOsm kg-1. These cells were sub-cultured every 2-3 days using standard procedure. Cells at exponential growth phase were harvested and used for in vitro cytotoxicity tests. 2.5. In vitro cytotoxicity assay using fish-derived cell lines SISS, SISK, IEE, IEK, IEG, SICH and ICG cells at exponential growth phase were collected and diluted to a concentration of 105 cells/ml in Leibovitzs L-15 medium with 10% FBS. After agitation, the cells were added to each well of 96-well tissue culture plates at the concentration of 2 x 104/well and incubated overnight at 28oC. After incubation, the medium was removed and the cells were re-fed with medium containing 0 (control), 10, 20, 30, 40 and 50 mg/L of chromium for 24 h EC50 analysis. Then four endpoints for cytotoxicity, i.e., MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay, Neutral red (NR) uptake assay, Alamar blue assay (AB) and protein concentration for Coomassie blue (CB) assay were determined after 24 h exposure as described by Borenfreund et al. (1988), Borenfreund and Puerner (1985), Taju et al. (2012) and Shopsis and Eng (1985), respectively. 2.5.1. Cell morphology SISS, SISSK, IEE, IEK, IEG, SICH and ICG cells were plated into a 24 well tissue culture plate at a density of 2ÃÆ'-105 cells (in 1 mL growth medium). After overnight growth, supernatants from the culture plates were removed and fresh aliquots of growth medium containing various concentrations of the chromium (0, 10, 20, 30, 40 and 50 mg/L) were exposed for 24 h. Upon incubation, cells were washed with phosphate-buffered saline (PBS, pH 7.4) and the morphological changes were observed under an inverted phase-contrast microscope (Carl Zeiss, Germany) at 100ÃÆ'- magnification. 2.6. Assessment of in vitro genotoxicity using fish-derived cell lines 2.6.1. Comet assay The Single Cell Gel Electrophoresis (comet assay) was performed on SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines according to the method of Singh et al. (1988) with slight modifications in accordance with the protocols of Taju et al. (2014). 5 x 104 cells on 500 ÃŽÂ ¼L of complete culture medium were seeded per well in a 24-well-plate. After 24 h incubation, cells were exposed to chromium using the following concentrations: 0 (control), 10, 20, 30, 40, 50 and 60 mg/L. At the end of the exposure period, cells were collected through trypsinization, followed by centrifugation at 1000 rpm for two minutes to obtain the pellet and avoid cell loss. After the centrifugations, the supernatant was discarded and the pellet resuspended in 100 ÃŽÂ ¼L of 0.9% agarose in milliQ water (low-melting point agarose, Sigma Aldrich chemicals, USA). The suspensions of cells in agarose were then applied dropwise to microscope slides containing an agarose layer (agarose electrophoresis grade, prep ared with a 1% concentration in milliQ water), and kept in a freezer for 10 min. The cells were lysed in freshly made lysing solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris-HCl, 10% DMSO, 1% Triton X-100, pH 10), for 1 h at 4 ÂÂ °C. After rinsing with redistilled water, the slides were placed on the horizontal gel box, covered with the cold alkaline buffer (0.3 M NaOH, 1 mM EDTA, pH >13) and left for 20 min. Electrophoresis was run in the same buffer at 25 V (0.83 V/cm) at 300 mA for 20 min at 4 ÂÂ °C. After electrophoresis the slides were neutralized in a cold neutralization buffer (0.4 M Tris-HCl, pH 7.5), for 2 to 5 min, fixed in methanol:acetic acid (3:1) for 5 min and stored in the dark at room temperature. Prior to examination, the slides were rehydrated and stained with 10 ÂÂ µg/mL ethidium bromide and examined using a Zeiss Axioplan epifluorescence microscope (Carl Zeiss, Germany). A positive control (5 ÂÂ µM H2O2) was also included in every batch of sample s. This strategy was chosen to compare the variation in the distance of migration. The positive control was not included in evaluation. Slides were examined at 100x magnifications using a fluorescence microscope. For each experimental condition 100 randomly chosen cells from two duplicate slides were examined (50 from each slide). In all 100 comets were scored visually according to the relative intensity of the fluorescence in the tail length. The extent of DNA migration was determined as a percentage of DNA in the tail (% tDNA) using an image analysis system comet 5, Kinetic Imaging Ltd. 2.6.2. Assessment Nuclear fragmentation by Hoechst 33258 Nuclear fragmentation of SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was analyzed with Hoechst 33258. The cells were seeded in 12-well cell culture plates and incubated overnight. Then the cells were treated with different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L). Cells were fixed in 4% paraformaldehyde in PBS for 30 min, washed with PBS, and stained with 1 ÃŽÂ ¼g/mL Hoechst 33258 in PBS for 30 min. Stained cells were washed twice with PBS. The changes in nuclei were observed with a fluorescent microscope through a UV filter. 2.7. Preparation of cell extract and Biochemical estimations The SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines were exposed to different concentrations of chromium (0, 10, 20, 30, 40, 50 and 60 mg/L) on 25 cm2 flasks for 24 h. After 24 h they were trypsinized and pelleted by centrifugation at 500ÃÆ'-g for 5 min. The cell pellet was washed with PBS (0.1M, pH7.4), resuspended in 500 ÂÂ µl chilled homogenizing buffer (250mM sucrose, 12mM Tris-HCl, 0.1mM DTT, pH 7.4) and lysed using Dounce homogenizer. The lysate was centrifuged (8000ÃÆ'-g, 10 min, 4 ÂÂ °C) and the supernatant (cell extract) was used for various biochemical assays. Protein concentration in the cell extract was estimated by the method of Lowry et al. (1951). The enzymatic antioxidant superoxide dismutase (SOD) activities were determined by following the procedures described by Kono (1978). Catalase (CAT) activity was determined by following the method described by Aebi (1974). The level of non-enzymatic antioxidant reduced glutathione (GSH) was estimated following the procedures described Saldak and Lindsay (1968). The activity of glutathione peroxidase (GPx) was assayed by the method of Flohe and Gunzler, (1984). The level of lipid peroxidation (LPO) was measured according to the method described Beuge and Aust (1978) based on the reaction with thiobarbituric acid. The results were recorded as ÂÂ µmol of TBA reactive substances/mg protein. The enzymatic and non-enzymatic parameters was expressed as ÂÂ µmol/mg protein. 2.8. Data analysis Experiments were performed in triplicate with eight replicates for each exposure concentration. Absolute values of each assay were transformed to control percentages. The results of LC50 and EC50 values were expressed as dilution in (mg/L) of the sample calculated using computerized (EPA, 2000) software. The individual data points of the concentration response cytotoxicity graph were presented as the arithmetic mean percent inhibition relative to the control standard error (SE). Cell viability and the concentration were fitted Scatter plots with the regressive equation (a linear regression model). The strength of the r2 value was used to determine whether a linear or quadratic relationship was assumed. Analysis of variance was used to determine whether groups of variables differed from each other (SPSS, Version 16). 3. Results The cumulative percentage mortality in L. calcarifer, E. suratensis and C. catla exposed to different concentrations of chromium was determined at 96 h and the results are presented in Fig 1. The toxic effect of chromium on the survival of fish was found to be concentration and time dependent. The chromium at the concentration of 50 mg/L caused 100%, 96.66% and 90% mortality, respectively, in L. calcarifer, E. suratensis and C. catla, whereas lower concentration of chromium at 10 mg/L caused 26.66%, 16.66% and 20% mortality of L. calcarifer, E. suratensis and C. catla respectively. No mortality was recorded in the control fish even after 96 h exposure. The LC50 values corresponding to 24, 48, 72 and 96 h of exposure of chromium were determined and results are presented in Table 1. Five different concentrations which ranged from 10 to 50 mg/L of chromium were used to carry out the in vitro toxicity assay in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines using four cytotoxicity end points (MTT, NR, AB and CB assays) and the results are shown in Fig.2 A-D. The cytotoxicity of chromium to SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was found to be similar in all the toxic endpoints employed. The lowest concentration of chromium tested (10 mg/L) was found to toxic in all the cell lines particularly SICH and IEK cell lines. The progressive increase in the concentration of chromium led to increase in toxicity when compared to control cells. The MTT, NR, AB and CB cytotoxicity endpoint assays revealed that a 24-h exposure of all the cell lines to different concentrations of chromium produced a dose-dependent reduction in the fraction of viability. The EC50 values and 95% confidence limit values obtained for chromium are summarized in Table 2. Correlations a mong the endpoints employed in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines to study cytotoxicity of chromium have been determined. A general tendency in the sensitivity among the four endpoints could be observed and statistical analysis revealed good correlation with R2 = 0.889-0.927 for all combinations between endpoints (Data not shown). The in vivo values of L. calcarifer vs. in vitro data of its two cell lines exposed to chromium were highly significant p2=0.956 (L. calcarifer vs. SISS) and 0.962 (L. calcarifer vs. SISK); R2=0.973 and 0.993; R2=0.980 and 0.975; R2=0.992 and 0.977 for MTT (Fig 3A), NR (Fig 3B), AB(Fig 3C) and CB (Fig 3D), respectively. The in vivo values of E. suratensis were compared with in vitro values of its three cell lines (IEE, IEG and IEK) exposed to chromium and were found to be highly significant p2=0.985 (E. suratensis vs. IEE), 0.987 (E. suratensis vs. IEK) and 0.968 (E. suratensis vs. IEG); R2=0.980, 0.936 and 0.956; R2=0.961, 0.955 and 0.904 and R2=0.955, 0.939 and 0.974 for MTT (Fig 3E), NR (Fig 3F), AB(Fig 3G) and CB (Fig 3H), respectively. Linear correlations between each in vitro vs. in vivo (C. catla)values of chromium were highly significant p2=0.991 (C. catla vs, SICH) and 0.993 (C. catla vs, ICG); R2=0.982 and 0.983; 0.974 and 0.990 and 0.987 and 0.984 for MTT (Fig 3I), NR (Fig 3J), AB(Fig 3K) and CB (Fig 3L), respectively. The prominent morphological changes of the cells exposed to high concentrations of chromium were observed. The changes observed include cell shrinkage, cell detachment, vacuolations and cell swelling in SISS (Fig 4H), SISK (Fig 4I), IEE (Fig 4J), IEK (Fig 4L), IEG (Fig 4L), SICH (Fig 4M) and ICG (Fig 4N) cell lines. In controls, no morphological alterations were observed in the SISS (Fig 4A), SISK (Fig 4B), IEE (Fig 4C), IEK (Fig 4D), IEG (Fig 4E), SICH (Fig 4F) and ICG (Fig 4G) cell lines. The percentage of DNA damage and the cumulative tail length from 100 cells per sample were measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 5. The length of tail DNA in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to 10 mg/L of chromium was estimated to be about 1.7%, 2.0%, 1.3%, 1.5%, 2.1%, 1.4% and 1.5%, respectively at a 24-h exposure, and chromium at the concentration of 50 mg/L caused 8.9%, 11.0%, 9.4%, 8.8%, 11.1%, 6.4% and 7.2% of tail DNA migration in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells, respectively (Fig. 5). Comet results of chromium exposed SISS, SISK, IEE, IEK, IEG, SICH and ICG cells showed a dose dependent increase in tail DNA (%) compared to the control cells, which gave the extent of DNA damage. The SISS, SISK, IEE, IEK, IEG, SICH and ICG cells were exposed to chromium for 24 h at different concentrations (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 6A-N. Apoptotic cells were identified by Hoechst staining of condensation and fragmentation of the nuclei as shown in SISS cells (Fig. 6H), SISK cells (Fig. 6I), IEE cells (Fig. 6J), IEK cells (Fig. 6K), IEG cells (Fig. 6L), SICH cells (Fig. 6M) and ICG cells (Fig. 6N) at higher concentration i.e. 50 mg/L of chromium exposed for 24 h, while no nuclear changes were observed in control cells are shown in SISS cells (Fig. 6A), SISK cells (Fig. 6B), IEE cells (Fig. 6C), IEK cells (Fig. 6D), IEG cells (Fig. 6E), SICH cells (Fig. 6F)and ICG cells (Fig. 6G). The level of antioxidant parameters such as SOD, CAT, GPx, GSH and LPO was measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium and the results were presented in Fig 7A-E. Regarding oxidative stress biomarkers, no significant change was observed in SOD, CAT, GSH and LPO levels in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to lower concentrations i.e. 10 mg/L of chromium when compared to the control cells. However, when these cell lines were exposed to 50 mg/L of chromium, the activity of SOD (~2.1, ~2.3, ~1.5, ~1.3, ~2.3, ~1.2 and ~2.2 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively in Fig 7A), CAT (~5.2, ~6.8, ~5.3, ~7.4, ~6.4, ~5.2 and ~4.6 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7B) and level GSH (~1.6, ~1.5, ~1.3, ~1.6, ~1.5, ~1.8 and ~1.3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7C) and GPx (~1.2, ~1.1, ~1.0, ~1.2, ~1.1, ~0.9 and ~1. 3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7D) decreased was found to be significantly (*P 4. Discussion Heavy metals constitute a main group of aquatic pollutants due to their bioacuumulative and non-biodegradable properties (Velma and Tchounwou 2010). Their excessive contamination of aquatic ecosystems has evoked major environmental and health concerns worldwide (Vutukuru et al. 2007). Chromium is the sixth most abundant heavy metal in the earth crust (U.S. EPA 1984). Fish and Fish cell lines constitute an excellent model to understand the mechanistic aspects of metal toxicity (Taju et al. 2014). In this study, we have examined the in vivo toxicity in three fish species in different environment i.e. L. calcarifer (Marine water), E. suratensis (brackish water) and C. catla (Fresh water), and in vitro cytotoxicity, oxidative stress and genotoxicity of the three same fish cell lines, SISS, SISK (Seabass spleen and kidney cell lines), IEE, IEK, IEG (Etroplus eye, kidney and gill cell lines), SICH and ICG (Catla heart and gill cell lines) an exposure to chromium. The results of this study clearly show that the fish cell lines experienced oxidative stress by modulating the antioxidant enzyme, exhibited DNA damage, nuclear fragmentation and microscopic morphological changes in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells. The LC50 values of chromium were determined as 30.22, 33.83 and 30.64 mg/L respectively in L. calcarifer, E. suratensis and C. catla, respectively at 96 h of exposure in this study. Recently, Mishra and Mohanty (2009) reported the LC50 values of chromium on Channa punctatus at 96 h of exposure as 41.75 mg/L. The LC50 values observed by Mishra and Mohanty (2009) were found to be higher when compared to L. calcarifer, E. suratensis and C. catla and this indicates that the L. calcarifer, E. suratensis and C. catla were found to more sensitive to chromium. Seven fish cell lines derived from L. calcarifer (SISS SISK), E. suratensis (IEE, IEK and IEG) and C. catla (SICH and ICG) were applied to evaluate the cytotoxicity of chromium using MTT, AB, NR and cell protein (CB) assays. The results of in vitro assays were compared with the results of in vivo test to determine the suitability of these fish cell lines for toxicological studies to replace the use of whole fish. The evaluation of cytotoxicity of chemical substances using animal cells has been carried out by many workers (Ekwall 1980a, 1983; Metcalfe 1971; Muir 1983a, 1983b; Paganuzzi et al. 1981; Benoit et al. 1987). Four commonly used endpoint assays (MTT, NR AB and cell protein assay CB) were employed in the present study using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensi, C. catla and L. calcarifer to evaluate the in vitro cytotoxicity of chromium. The main observation was that the cytotoxicity was closely associated in all the seven cell lines independent of the toxic endpoints employed. This not only supports the observations of Ekwall (1995) and Li and Zhang (2002) that most cell lines have a similar results to toxicants when toxicity is measured by different endpoints, corresponding to inhibition or destruction of basal functions and structures, and also suggests that endpoints employed in the present study can also be used to predict acute cytotoxicity. Tan et al. (2008) have used six fish cell lines to study the toxicity of four heavy metals: cadmium, chromium, zinc, and copper by using two cytotoxicity endpoints MTT and CB assays. The results revealed that carp epithelioma cells are least tolerant to chromium. The NR uptake assay is a useful method for comparing the relative acute cytotoxicity of metals in vitro with metal and chemicals toxicity studies in whole fish in vivo (Brandao et al. 1992; Ryan and Hightower 1994; Taju et al. 2013). In the present study, we employed that SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines for cytotoxicity assessment of chromium by using four endpoints. Our results show that there is no significant difference between all the four endpoints. Segner (1994) reported that the relationship of the in vitro cytotoxicity values to in vivo fish toxicity data is less satisfying and that this might be due to the inconsistency of the in vivo values. As observed in the present study, a positive relationship of acute lethal potency in fish with in vitro cytotoxicity has been found by Fry et al. (1990). Castano et al. (1996) found good correlations between in vivo and in vitro for each endpoint and for the cytotoxicity index and suggested the applicability of the RTG-2 cell line as an alternative protocol to estimate the acute toxicity of chemicals on fish without using live animals. The correlation of in vitro cytotoxicity of metals with in vivo toxicity data was evaluated by comparing the 24 h NR50 results of R1 cells to 96 h LC50 data of different fish species. The rvalues (R1 cell line) were 0.64 for the relation between LC50, data of golden ide and bluegill sunfish, 0.58 for golden ide and rainbow trout in soft water, and 0.68 for golden ide and rainbow trout in hard water (Segner et al. 1994). In the present study, in vitro cytotoxicity of chromium with in vivo results was evaluated by comparing the 24 h MTT, NR, AB and CB data of seven Indian fish cell lines to 96 h data of three fish species (L. calcarifer, E. suratensis and C. catla). A good correlation was found between in vitro of seven fish cell lines compared with in vivo values of whole fish exposed to chromium for 24 h and 96 h respectively, with r=0.902 to 0.99. The results revealed that the four endpointsvalues were closely correlated to whole fish in vivo values and that the linear correlation b etween each in vitro parameter and the in vivo data were found to be highly significant. The results of in vitro assays using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensis, C. catla and L. calcarifer were correlated with those obtained from in vivo assay using the same species of fish (L. calcarifer, E. suratensis and C. catla). Based on the results of the present study we recommend the use of these seven cell lines instead of living fish for toxicity assessment of metal salts and environmental contaminants. The present study showed that chromium induced genotoxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines by comet assay. DNA damage was observed in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines exposed to chromium in a concentration dependent manner. The DNA damage at higher test concentrations in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines could be due to the elevated levels of tail DNA in all cell lines compared to their controls cells. Induction of ROS under metallic stress could attack the DNA and damage its integrity. Our present results are similar to the previous reports (Iqbal Ahmad et al. 2006; Velma and Tchounwou 2010; 2013) DNA damage in gill and kidney of Anguilla anguilla L. exposed to chromium with or without pre-exposure to ÃŽÂ ²-naphthoflavone. In another study, medaka fin cell lines exposed to Cr (VI) to examine the genotoxic potentials, have observed DNA double strand breaks a

The F.B.I. Essay examples -- FBI Police Crime Essays

The F.B.I. To uphold the law through the investigation of violations of federal criminal law; to protect the U.S. from foreign intelligence and terrorist activities; to provide leadership and law enforcement assistance to federal, state, local, and international agencies; and to perform these responsibilities in a manner that is responsive to the needs of the public and is faithful to the constitution of the U.S.: this is the mission of the Federal Bureau of Investigation. The agency now known as the Federal Bureau of Investigation was founded in 1908 when the Attorney General appointed an unnamed force of Special Agents to be the investigative force of the Department of Justice (DOJ). Before that time, the DOJ had to borrow Agents from the U.S. Secret Service to investigate violations of federal criminal laws within its jurisdiction. In 1909, the Special Agent Force was renamed the Bureau of Investigation, and after a series of name changes, it received its present official name in 1935. During the early period of the FBIs history, its agents investigated violations of mainly bankruptcy frauds, antitrust crime, and neutrality violation. During World War One, the Bureau was given the responsibility of investigating espionage, sabotage, sedition (resistance against lawful authority), and draft violations. The passage of the National Motor Vehicle Theft Act in 1919 further broadened the Bureau's jurisdiction. After the passage of Prohibition in 1920, the gangster era began, bringing about a whole new type of crime. Criminals engaged in kidnapping and bank robbery, which were not federal crimes at that time. This changed in 1932 with the passage of a federal kidnapping statute. In 1934, many other federal criminal statutes were passed, and Congress gave Special Agents the authority to make arrests and to carry firearms. The FBIs size and jurisdiction during the second World War increased greatly and included intelligence matters in South America. With the end of that war, and the arrival of the Atomic Age, the FBI began conducting background security investigations for the White House and other government agencies, as well as probes into internal security matters for the executive branch of the government. In the 1960s, civil rights and organized crime became major concerns of the FBI, and counterterrorism, drugs, financial crime, and v... .... hold a bachelors degree obtained in an accredited four-year resident program at a college or university; and 4. have three years full-time work experience, or fluency in a language for which the Bureau has a need for. After graduation from the FBI Academy, a new Special Agent is assigned to an FBI field office. This assignment is determined by the individuals special skills and the needs of the bureau. As part of their duties, Special Agents are required to relocate during their careers. Special Agents enter service in Grade GS 10 on the federal governments General Schedule pay scale and can advance to Grade 13 in field assignment. In our society today, one of the most important things to us is our safety. Organizations like the FBI help protect us and investigate crimes to help prevent future ones. Their motto is Fidelity, Bravery, and Integrity, and I think that each one of those words is justified when it comes to describing the Federal Bureau of Investigation. When the duties of the FBI are stated in the mission it says to perform these duties in a manner that is responsive to the needs of the public and is faithful to the Constitution of the United States.

Coca-Cola entering a new market :: essays research papers

Introduction To give a short introduction to the circumstances affecting this case of Pepsi & Coca Cola it has to be said that in general it is not just simple for MNEs to invest and enter foreign markets as regulations and restrictions differ from coutry to country and hence ifluence international business negotiations to a great extend. Therefore MNEs investigating in foreign markets have to either adopt to those condition given by the host country government, which of course to a certain extend has to be negotiated as no one of those parties want to loose their maximum independence- or the MNE decides not to take further steps towards the foreign operation and leaves the feeld by assumingly – in turn – missing out a great opportunity, but this again depends on a complexity of economic and cultural reasons influencing international trade, which I will develop critically in the further case study of Pepsi & Coke in accordance to the following questions. 1.)  Ã‚  Ã‚  Ã‚  Ã‚  Did PepsiCo make too many concessions in order to enter the Indian market? Could the company have negotiated better? In this case study PepsiCo – for the second time – intends to enter the Indian market, though already having experienced major problems which consequently led to their first departure (for non profitability). As well Coca Cola departured India after harsh disagreements with the government. Why after all did Pepsi enter again, facing a country with such strongly adverse feelings towards foreign companies – which is rooted in Indians history of colonialistic times when the British, French and Portuguese were extracting the country‘s recources ‚‘its wealth‘ without returning noticeable benefits to its economy. Moreover they feared that national companies would not be able to compete with foreign investors and as a result of this high artificially prices and profit margins reduced incentives for national companies to enter. This almost irrepairable bad approach towards foreigner went even that far that journalist widely reported that PepsiCo had a CIA connection aimed at undermining India‘s independence. However returning to the argument of PepsiCo having too many concessions or not, first as should have become clear now, the company was confronted with a govvernmental volatility and unwillingness to negotiate. It was rather a one-way game wherein PepsiCo had to agree with completely, or take its departure, as the company was not only faced with economic but also with moral issues (as mentioned above). Especially the confidence factor plays a great part in her which for the company turned out to be a rather costly factor as PepsiCo had to make various concession before they could enter the Indian market.

How you can enjoy your vacation on your budget Essay

From time to time people like to take vacation and enjoy their time in quiet and peaceful places. Then they start to plan to their vacation by choosing the season, place and collecting the information about it. In addition, to the cost of that vacation, the budget is a very important issue to decide the possible destination. Every one looks to the dream vacation but we have to adapt it to our budget. The people with a low budget have limited choices or ability to enjoy their vacation in their countries. On the other hand the people with a high budget can visit variety of places. The vacation will be affected directly by type of budget: low budget, medium budget, and high budget. The first group includes those who have low budgets and would like to spend their vacation in Amazing places but they face the fact that their budget does not allow them to go there. Nevertheless, they can enjoy in their time even they have a low budget. The people who live in Toronto with a low budget cannot travel to Mexico, or even some states in the US, but they can enjoy a lot of places in Ontario, other provinces, visit new places in Canada and match their activities to their budget like camping, fishing or any other activities. Also we can spend our days cheaply. Walk around a lot, and visit free or cheap attractions. Use shuttle buses or other cheap public transport. We can learn a lot about a place without spending a lot of money. The next group, those who have a medium budget can go little bit further like US and South America. We have to look at all details, like the airfare and hotel accommodations and choose the best options that you can take that would still allow you to get to your destination. We can research for good accommodation deals online and get lists of all the hostels, motels, and hotels in the place that we have selected to visit. We have to remember that the accommodations do not necessarily need to be expensive as we are just looking for a place to sleep and save money to spend on our self like restaurants, attractions, and all other things. Medium budget give us more choices and variety to enjoy in our vacation. The last group includes those who have high budgets which can travel a long distance and have the ability to visit different cities or  countries and spend a lot of money. They can enjoy a trip in a grand and elegant way by spending quite a handsome amount of money to rest in a great hotel, to have an exclusive meal and to enjoy top- class service. Some people like to have luxury and visit some countries like France and Italy and enjoy in all luxury services. They can stay in a five star hotel like Hotel De Crillon in Paris. In addition, they visit the most famous places like the Louvre museum and the Eiffel tower. The vacation with a high budget gives you the ability to do a wide variety of things. To concludes we have to enjoy in our vacation by planning the target place which will match Our budget. We spend our vacation in our country like Canada, travel little bit further like United States and South America, or travel to long distance places like Italy and France. We have to plan Very well and enjoy our time without thinking about luxury places if we are not able to visit that place. There is no reason to prevent us from having fun in our vacation whatever our budget is, low, medium, or high.